Development and validation of a rapid resolution liquid chromatographic (RRLC) method for the determination of Atomoxetine HCl in its pharmaceutical dosage form

The present work described the development of a rapid resolution liquid chromatographic (RRLC) method for atomoxetine in its pharmaceutical dosage form Strattera ®. analysis was achieved on Agilent Eclipse XDB C18 column (50 mm x 4.6 i.d, 1.8 particle size) using mixture aqueous 0.04 M Glacial acetic acid and 0.03M triethylamine (TEA), pH - Acetonitril (42 : 58, v/v) as mobile phase at flow rate 0.27 ml/min UV detection 220 nm. developed linear over concentration range 4-40 mg /ml (r = 0.99969) with limit quantitation 0.44 1.32 atomoxetine. RRLC validated respect to specificity, linearity, accuracy, precision, quantitation. statistical proved that quantification bulk drug from preparation is reproducible selective. proposed can be used fo quality control formulated products containing Rapid chromatography has become an increasingly useful approach achieve higher throughput, improve sensitivity reduce costs. agilent 1200 LC system enables faster (theoretically up 20x) than conventional high performance (HPLC) while maintaining equivalent resolution. This by sub-2 micron chemistry.